Hotspot

ycf1-trnN-GUU

IRa · 127735-129910 · 2176 bp

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Pi 0.222
MarkerSeek score 81.40
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
2176
variable_sites
446
pi
0.222
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 TATGGTATCCCAGGCCTCTG CCCCGTAACTCTTCTTCAGC 55.0 55.0 58.0 58.0 2953 bp 0.5 59.7
ycf1-trnN-GUU_p2 TATGGTATCCCAGGCCTCTG CCCGTAACTCTTCTTCAGCC 55.0 55.0 58.0 58.0 2952 bp 0.5 59.7
ycf1-trnN-GUU_p3 TATGGTATCCCAGGCCTCTG GCCCCGTAACTCTTCTTCAG 55.0 55.0 58.0 58.0 2954 bp 0.5 59.7
ycf1-trnN-GUU_p4 GTATGGTATCCCAGGCCTCT CCCCGTAACTCTTCTTCAGC 55.0 55.0 58.0 58.0 2954 bp 0.5 59.7
ycf1-trnN-GUU_p5 GTATGGTATCCCAGGCCTCT CCCGTAACTCTTCTTCAGCC 55.0 55.0 58.0 58.0 2953 bp 0.5 59.7