Hotspot

ndhG-ndhI

SSC · 119250-119611 · 362 bp

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Pi 0.433
MarkerSeek score 76.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
362
variable_sites
138
pi
0.433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 CCCAACGGTCCAAAGAGAAA TGATTACCTTGTCTCACGCG 50.0 50.0 58.0 58.0 1063 bp 0.5 59.7
ndhG-ndhI_p2 TGATCCCATACCACGACGTA TGATTACCTTGTCTCACGCG 50.0 50.0 57.9 58.0 1145 bp 0.5 59.5
ndhG-ndhI_p3 CGCAGCAGCTACAAAATACG TGATTACCTTGTCTCACGCG 50.0 50.0 57.9 58.0 958 bp 0.5 59.3
ndhG-ndhI_p4 CCCAACGGTCCAAAGAGAAA ACATCAGAACGTTTCCGAGG 50.0 50.0 58.0 57.9 998 bp 0.5 59.2
ndhG-ndhI_p5 TGATCCCATACCACGACGTA ACATCAGAACGTTTCCGAGG 50.0 50.0 57.9 57.9 1080 bp 0.5 58.9