Hotspot

rpl16

IRb · 82966-84414 · 1449 bp

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Pi 0.0142

MarkerSeek score 60.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: IRb
length_bp: 1449
variable_sites: 67
pi: 0.0142
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTCTGGCTCCGCTGTTATC	AAACCCTTACGGACACCCTA	50.0	50.0	58.0	58.0	2046-2092 bp	1.0	84.8
rpl16_p2	TTTCTGGCTCCGCTGTTATC	AACCCTTACGGACACCCTAA	50.0	50.0	58.0	58.0	2045-2091 bp	1.0	84.8
rpl16_p3	CCATTGGTGCGTTTGAGTTC	AAACCCTTACGGACACCCTA	50.0	50.0	57.9	58.0	2214-2260 bp	1.0	84.4
rpl16_p4	CCATTGGTGCGTTTGAGTTC	AACCCTTACGGACACCCTAA	50.0	50.0	57.9	58.0	2213-2259 bp	1.0	84.4
rpl16_p5	CTGTCTTAATTCCCGGGCAA	AACCCTTACGGACACCCTAA	50.0	50.0	57.9	58.0	2316-2362 bp	1.0	83.7