Hotspot

ndhF-rpl32

SSC · 114097-115275 · 1179 bp

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Pi 0.0252

MarkerSeek score 66.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1179
variable_sites: 93
pi: 0.0252
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATCTTTTGTTGCC	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.0	57.8	1334-1399 bp	1.0	88.2
ndhF-rpl32_p2	CCAGAATTCCCACCGTAGTG	AAAAGGCTTTCAATGCTGCC	55.0	45.0	58.0	57.8	1541-1606 bp	1.0	87.8
ndhF-rpl32_p3	CGCGATTAGTTACAAACGCC	AAAAGGCTTTCAATGCTGCC	50.0	45.0	57.9	57.8	1772-1837 bp	1.0	86.8
ndhF-rpl32_p4	ACAAACGCCTTTTGACAAGC	AAAAGGCTTTCAATGCTGCC	45.0	45.0	58.1	57.8	1761-1824 bp	0.875	79.6
ndhF-rpl32_p5	CAAACGCCTTTTGACAAGCA	AAAAGGCTTTCAATGCTGCC	45.0	45.0	58.1	57.8	1760-1823 bp	0.875	79.6