Hotspot

rpl36-rps8

IRb · 81410-81924 · 515 bp

Back to Boehmeria

Pi 0.0147

MarkerSeek score 61.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: IRb
length_bp: 515
variable_sites: 28
pi: 0.0147
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACGGTGTCCCTCTTCTTGTA	TTGCCCGGGAATTAAGACAG	50.0	50.0	58.0	57.9	1589-1612 bp	0.875	80.6
rpl36-rps8_p2	ACGGTGTCCCTCTTCTTGTA	GCCCGGGAATTAAGACAGTT	50.0	50.0	58.0	57.9	1587-1610 bp	0.875	80.6
rpl36-rps8_p3	ACGGTGTCCCTCTTCTTGTA	TTAGGTGGGATGGGGATTGT	50.0	50.0	58.0	58.0	1034-1061 bp	0.875	80.4
rpl36-rps8_p4	AGGACCATGAAATTACCCGC	TTAGGTGGGATGGGGATTGT	50.0	50.0	57.9	58.0	989-1016 bp	0.875	80.2
rpl36-rps8_p5	TGCAACCGTTATGTGGCATA	TTAGGTGGGATGGGGATTGT	45.0	50.0	57.9	58.0	1217-1244 bp	0.875	79.0