Hotspot

rps4-trnT-UGU

LSC · 48020-48543 · 524 bp

Back to Boehmeria

Pi 0.00922
MarkerSeek score 62.00
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
524
variable_sites
18
pi
0.00922
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CCCCCAGACGACGTATTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.9 57.9 596-647 bp 1.0 84.4
rps4-trnT-UGU_p2 CCCCCAGACGACGTATTTTT GATGGTCATCGGTTCGACTC 50.0 55.0 57.9 58.2 565-616 bp 1.0 84.3
rps4-trnT-UGU_p3 GAAACGAGGCCCTCTGTAAC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.3 57.9 574-625 bp 1.0 83.1
rps4-trnT-UGU_p4 GAAACGAGGCCCTCTGTAAC GATGGTCATCGGTTCGACTC 55.0 55.0 58.3 58.2 543-594 bp 1.0 83.0
rps4-trnT-UGU_p5 TTTTTGAAACGAGGCCCTCT CTCAGAGGTTAGAGCATCGC 45.0 55.0 57.6 57.9 579-630 bp 1.0 82.4