Hotspot

trnT-GGU-psbD

LSC · 33138-34547 · 1410 bp

Back to Boehmeria

Pi 0.0128
MarkerSeek score 64.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1410
variable_sites
49
pi
0.0128
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AGTAGACCGGACCAACCTAC 55.0 55.0 58.1 58.2 1339-1579 bp 1.0 84.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA AGTAGACCGGACCAACCTAC 50.0 55.0 58.1 58.2 1342-1582 bp 1.0 84.7
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1321-1561 bp 1.0 84.2
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1335-1575 bp 1.0 84.0
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1324-1564 bp 1.0 84.0