Hotspot

trnC-GCA-petN

LSC · 29149-30344 · 1196 bp

Back to Boehmeria

Pi 0.0134

MarkerSeek score 65.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 49
pi: 0.0134
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCGGATTTGAACTGGGGAAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1013-1366 bp	1.0	85.2
trnC-GCA-petN_p2	CCGGATTTGAACTGGGGAAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1074-1427 bp	1.0	83.5
trnC-GCA-petN_p3	AAAAGGATTTGCAGTCCCCC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	995-1348 bp	1.0	83.1
trnC-GCA-petN_p4	AAAAGGATTTGCAGTCCCCC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	1056-1409 bp	1.0	81.3
trnC-GCA-petN_p5	TAATTAGGCGACACCCGGAT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.6	58.2	1027-1380 bp	1.0	81.2