Hotspot

psbK-psbI

LSC · 8945-9434 · 490 bp

Back to Boehmeria

Pi 0.0129

MarkerSeek score 62.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 21
pi: 0.0129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	594-619 bp	1.0	85.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	595-620 bp	1.0	85.8
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	599-624 bp	1.0	85.7
psbK-psbI_p4	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	600-625 bp	1.0	85.7
psbK-psbI_p5	CTTTTGTTTGGCAAGCTGCT	TTACGCCCCGGATCATTAGA	45.0	50.0	58.1	58.3	601-626 bp	1.0	83.9