Hotspot

trnH-GUG-psbA

LSC · 1431-1715 · 285 bp

Back to Boehmeria

Pi 0.0424

MarkerSeek score 69.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 285
variable_sites: 32
pi: 0.0424
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TAGGCTGGTTCCTATAGGCC	GAACTTAGCTTCCGTCTGGG	55.0	55.0	58.0	58.0	1428-1495 bp	0.25	51.2
trnH-GUG-psbA_p2	TAGGCTGGTTCCTATAGGCC	GGAACTTAGCTTCCGTCTGG	55.0	55.0	58.0	58.0	1429-1496 bp	0.25	51.2
trnH-GUG-psbA_p3	GCCTTGACCTACTTGGCTAC	GAACTTAGCTTCCGTCTGGG	55.0	55.0	58.0	58.0	981 bp	0.125	44.7
trnH-GUG-psbA_p4	GCCTTGACCTACTTGGCTAC	GGAACTTAGCTTCCGTCTGG	55.0	55.0	58.0	58.0	982 bp	0.125	44.7
trnH-GUG-psbA_p5	GCCTTGACCTACTTGGCTAC	GGGAACTTAGCTTCCGTCTG	55.0	55.0	58.0	58.0	983 bp	0.125	44.7