primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TAGGCTGGTTCCTATAGGCC	GAACTTAGCTTCCGTCTGGG	20	20	55.0	55.0	58.0	58.0	14.5	0.0	0.037176	1431	1715	1428-1495	0.250000	51.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TAGGCTGGTTCCTATAGGCC	GGAACTTAGCTTCCGTCTGG	20	20	55.0	55.0	58.0	58.0	14.5	0.0	0.037176	1431	1715	1429-1496	0.250000	51.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GCCTTGACCTACTTGGCTAC	GAACTTAGCTTCCGTCTGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.037129	1431	1715	981-981	0.125000	44.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GCCTTGACCTACTTGGCTAC	GGAACTTAGCTTCCGTCTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.037129	1431	1715	982-982	0.125000	44.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GCCTTGACCTACTTGGCTAC	GGGAACTTAGCTTCCGTCTG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.037129	1431	1715	983-983	0.125000	44.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.173044	5697	6490	865-942	1.000000	87.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.173044	5697	6490	864-941	1.000000	87.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	TCAAAGAAGGCGGGTGTTTT	20	20	50.0	45.0	58.2	58.2	20.3	0.0	0.398125	5697	6490	861-938	1.000000	85.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCAAAGAAGGCGGGTGTTT	20	20	50.0	45.0	58.2	58.2	20.3	0.0	0.398125	5697	6490	862-939	1.000000	85.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TTTCAAAGAAGGCGGGTGTT	20	20	50.0	45.0	58.2	58.2	20.3	0.0	0.398125	5697	6490	863-940	1.000000	85.9
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	8945	9434	594-619	1.000000	85.8
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	8945	9434	595-620	1.000000	85.8
psbK-psbI_p3	psbK-psbI	3	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.122947	8945	9434	599-624	1.000000	85.7
psbK-psbI_p4	psbK-psbI	4	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.122947	8945	9434	600-625	1.000000	85.7
psbK-psbI_p5	psbK-psbI	5	CTTTTGTTTGGCAAGCTGCT	TTACGCCCCGGATCATTAGA	20	20	45.0	50.0	58.1	58.3	15.2	0.7	0.348862	8945	9434	601-626	1.000000	83.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	CCGGATTTGAACTGGGGAAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	29149	30344	1013-1366	1.000000	85.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	CCGGATTTGAACTGGGGAAA	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	29149	30344	1074-1427	1.000000	83.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	AAAAGGATTTGCAGTCCCCC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.6	0.0	0.506839	29149	30344	995-1348	1.000000	83.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	AAAAGGATTTGCAGTCCCCC	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.6	0.0	0.706353	29149	30344	1056-1409	1.000000	81.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	TAATTAGGCGACACCCGGAT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.6	58.2	0.7	0.0	0.735105	29149	30344	1027-1380	1.000000	81.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	32412	33065	723-758	1.000000	85.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	32412	33065	721-756	1.000000	85.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32412	33065	777-812	1.000000	84.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	32412	33065	702-737	1.000000	84.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	32412	33065	700-735	1.000000	84.8
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	AGTAGACCGGACCAACCTAC	20	20	55.0	55.0	58.1	58.2	7.6	9.5	0.219381	33138	34547	1339-1579	1.000000	84.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	AGTAGACCGGACCAACCTAC	20	20	50.0	55.0	58.1	58.2	0.0	9.5	0.241535	33138	34547	1342-1582	1.000000	84.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33138	34547	1321-1561	1.000000	84.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.323334	33138	34547	1335-1575	1.000000	84.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33138	34547	1324-1564	1.000000	84.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	57.9	58.1	4.1	11.5	0.219123	43483	44261	884-983	1.000000	83.8
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCGGGTAAT	20	20	50.0	50.0	57.9	58.3	4.1	18.7	0.419280	43483	44261	912-1011	1.000000	82.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TGAAGCGCAGAATTGGTTGA	20	20	50.0	45.0	57.9	58.4	4.1	11.5	0.511886	43483	44261	887-986	1.000000	81.4
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGAAGCGCAGAATTGGTTG	20	20	50.0	45.0	57.9	57.6	4.1	11.5	0.535971	43483	44261	888-987	1.000000	81.2
psaA-ycf3_p5	psaA-ycf3	5	TGTTCTTGAGAAATGGCCCG	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	58.5	58.1	0.0	11.5	0.570155	43483	44261	909-1008	1.000000	80.8
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.236277	48020	48543	596-647	1.000000	84.4
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CCCCCAGACGACGTATTTTT	GATGGTCATCGGTTCGACTC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.262088	48020	48543	565-616	1.000000	84.3
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.3	57.9	9.5	0.0	0.409520	48020	48543	574-625	1.000000	83.1
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GAAACGAGGCCCTCTGTAAC	GATGGTCATCGGTTCGACTC	20	20	55.0	55.0	58.3	58.2	9.5	0.0	0.435331	48020	48543	543-594	1.000000	83.0
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	20	20	45.0	55.0	57.6	57.9	9.5	0.0	0.491062	48020	48543	579-630	1.000000	82.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	23	20	34.8	55.0	56.1	58.7	0.0	0.0	5.564602	52634	53129	524-584	1.000000	48.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	56.1	57.8	0.0	0.0	5.078536	52634	53129	536-596	1.000000	47.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCCAGAAAATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	23	20	34.8	55.0	56.1	57.5	0.0	0.0	5.422308	52634	53129	535-595	1.000000	47.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ATGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.6	57.8	0.0	0.0	5.595649	52634	53129	537-597	1.000000	47.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCCAGAAAATATCATATTCGT	AAGGTCTACGGTTCGAGTCC	23	20	34.8	55.0	56.1	58.8	0.0	0.0	5.709077	52634	53129	534-594	1.000000	47.9
rpl36-rps8_p1	rpl36-rps8	1	ACGGTGTCCCTCTTCTTGTA	TTGCCCGGGAATTAAGACAG	20	20	50.0	50.0	58.0	57.9	0.0	29.3	0.128153	81410	81924	1589-1612	0.875000	80.6
rpl36-rps8_p2	rpl36-rps8	2	ACGGTGTCCCTCTTCTTGTA	GCCCGGGAATTAAGACAGTT	20	20	50.0	50.0	58.0	57.9	0.0	29.3	0.129647	81410	81924	1587-1610	0.875000	80.6
rpl36-rps8_p3	rpl36-rps8	3	ACGGTGTCCCTCTTCTTGTA	TTAGGTGGGATGGGGATTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010971	81410	81924	1034-1061	0.875000	80.4
rpl36-rps8_p4	rpl36-rps8	4	AGGACCATGAAATTACCCGC	TTAGGTGGGATGGGGATTGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.058384	81410	81924	989-1016	0.875000	80.2
rpl36-rps8_p5	rpl36-rps8	5	TGCAACCGTTATGTGGCATA	TTAGGTGGGATGGGGATTGT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.120944	81410	81924	1217-1244	0.875000	79.0
rpl16_p1	rpl16	1	TTTCTGGCTCCGCTGTTATC	AAACCCTTACGGACACCCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052737	82966	84414	2046-2092	1.000000	84.8
rpl16_p2	rpl16	2	TTTCTGGCTCCGCTGTTATC	AACCCTTACGGACACCCTAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052737	82966	84414	2045-2091	1.000000	84.8
rpl16_p3	rpl16	3	CCATTGGTGCGTTTGAGTTC	AAACCCTTACGGACACCCTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079038	82966	84414	2214-2260	1.000000	84.4
rpl16_p4	rpl16	4	CCATTGGTGCGTTTGAGTTC	AACCCTTACGGACACCCTAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079038	82966	84414	2213-2259	1.000000	84.4
rpl16_p5	rpl16	5	CTGTCTTAATTCCCGGGCAA	AACCCTTACGGACACCCTAA	20	20	50.0	50.0	57.9	58.0	18.5	0.0	0.151345	82966	84414	2316-2362	1.000000	83.7
ndhF-rpl32_p1	ndhF-rpl32	1	GGCGAAGATCTTTTGTTGCC	AAAAGGCTTTCAATGCTGCC	20	20	50.0	45.0	58.0	57.8	9.9	13.8	0.191751	114097	115275	1334-1399	1.000000	88.2
ndhF-rpl32_p2	ndhF-rpl32	2	CCAGAATTCCCACCGTAGTG	AAAAGGCTTTCAATGCTGCC	20	20	55.0	45.0	58.0	57.8	0.6	13.8	0.198539	114097	115275	1541-1606	1.000000	87.8
ndhF-rpl32_p3	ndhF-rpl32	3	CGCGATTAGTTACAAACGCC	AAAAGGCTTTCAATGCTGCC	20	20	50.0	45.0	57.9	57.8	0.0	13.8	0.241823	114097	115275	1772-1837	1.000000	86.8
ndhF-rpl32_p4	ndhF-rpl32	4	ACAAACGCCTTTTGACAAGC	AAAAGGCTTTCAATGCTGCC	20	20	45.0	45.0	58.1	57.8	11.5	13.8	0.253120	114097	115275	1761-1824	0.875000	79.6
ndhF-rpl32_p5	ndhF-rpl32	5	CAAACGCCTTTTGACAAGCA	AAAAGGCTTTCAATGCTGCC	20	20	45.0	45.0	58.1	57.8	0.0	13.8	0.254445	114097	115275	1760-1823	0.875000	79.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCATTGAAAGCCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.182194	115435	116222	817-964	1.000000	87.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCATTGAAAGCCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.183564	115435	116222	818-965	1.000000	87.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCATTGAAAGCCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.298641	115435	116222	827-974	1.000000	86.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCAGCATTGAAAGCCTTTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.370658	115435	116222	826-973	1.000000	85.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCATTGAAAGCCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.370658	115435	116222	825-972	1.000000	85.6
ccsA-ndhD_p1	ccsA-ndhD	1	ACGAAATAACCACGCCAAGT	TGACAAAGTCGAAGCCCTTC	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.234160	117335	117623	998-1010	1.000000	82.8
ccsA-ndhD_p2	ccsA-ndhD	2	ACGAAATAACCACGCCAAGT	ATGGTAATGCCCCAAACTCC	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.272293	117335	117623	1122-1134	1.000000	82.4
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAAATAACCACGCCAAGT	GACAAAGTCGAAGCCCTTCT	20	20	45.0	50.0	58.1	57.8	0.0	6.5	0.284454	117335	117623	997-1009	1.000000	82.4
ccsA-ndhD_p4	ccsA-ndhD	4	ACGAAATAACCACGCCAAGT	CTTGGTTGATGATGGTCGGT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.226974	117335	117623	1632-1644	1.000000	81.8
ccsA-ndhD_p5	ccsA-ndhD	5	ACGAAATAACCACGCCAAGT	GCGTATGGATTGGTTCGGAT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.234160	117335	117623	1693-1705	0.875000	76.4
ycf1_p1	ycf1	1	CTTCGGACCATTCGGGAAAT	TGGAAGACAAAAGTTGCATACA	20	22	50.0	36.4	58.0	56.5	0.0	0.0	3.568430	129680	129799	1005-1050	1.000000	60.3
ycf1_p2	ycf1	2	ACGCCGTTATAGGTGCATTT	TGGAAGACAAAAGTTGCATACA	20	22	45.0	36.4	58.0	56.5	0.0	0.0	3.562723	129680	129799	1057-1102	1.000000	60.0
ycf1_p3	ycf1	3	AACGCCGTTATAGGTGCATT	TGGAAGACAAAAGTTGCATACA	20	22	45.0	36.4	58.0	56.5	0.0	0.0	3.562723	129680	129799	1058-1103	1.000000	59.9
ycf1_p4	ycf1	4	AGATTTGTGCCAAGGCTTCA	TGGAAGACAAAAGTTGCATACA	20	22	45.0	36.4	58.0	56.5	13.0	0.0	3.530394	129680	129799	1154-1199	1.000000	59.7
ycf1_p5	ycf1	5	ATTTGTGCCAAGGCTTCAGA	TGGAAGACAAAAGTTGCATACA	20	22	45.0	36.4	58.0	56.5	13.0	0.0	3.530394	129680	129799	1152-1197	1.000000	59.7
rps8_p1	rps8	1	GAACTCAAACGCACCAATGG	ACGTCTGCCGGTTGAATTAT	20	20	50.0	45.0	57.9	57.6	0.0	1.9	0.447067	159870	160274	1084-1104	0.250000	50.4
rps8_p2	rps8	2	GAACTCAAACGCACCAATGG	ATACGTCTGCCGGTTGAATT	20	20	50.0	45.0	57.9	57.6	0.0	1.9	0.447067	159870	160274	1086-1106	0.250000	50.4
rps8_p3	rps8	3	TTGCCCGGGAATTAAGACAG	ACGTCTGCCGGTTGAATTAT	20	20	50.0	45.0	57.9	57.6	29.3	1.9	0.519373	159870	160274	981-1001	0.250000	50.2
rps8_p4	rps8	4	TTGCCCGGGAATTAAGACAG	ATACGTCTGCCGGTTGAATT	20	20	50.0	45.0	57.9	57.6	29.3	1.9	0.519373	159870	160274	983-1003	0.250000	50.2
rps8_p5	rps8	5	GCCCGGGAATTAAGACAGTT	ACGTCTGCCGGTTGAATTAT	20	20	50.0	45.0	57.9	57.6	29.3	1.9	0.520867	159870	160274	979-999	0.250000	50.2