Hotspot

rps15-ycf1

SSC · 124030-127473 · 3444 bp

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Pi 0.0102

MarkerSeek score 52.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 3444
variable_sites: 76
pi: 0.0102
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAAAAGAAATCCTTCCCTTTTT	GAACCTGTCCGTTTGTCTGT	27.3	50.0	52.6	58.1	843-857 bp	1.0	43.7
rps15-ycf1_p2	AAAAAGAAATCCTTCCCTTTTT	TGAACCTGTCCGTTTGTCTG	27.3	50.0	52.6	58.1	844-858 bp	1.0	43.7
rps15-ycf1_p3	AAAAAGAAATCCTTCCCTTTTT	CCTGTCCGTTTGTCTGTACA	27.3	50.0	52.6	57.5	840-854 bp	1.0	43.7
rps15-ycf1_p4	AAAAAGAAATCCTTCCCTTTTT	ACCTGTCCGTTTGTCTGTAC	27.3	50.0	52.6	57.5	841-855 bp	1.0	43.7
rps15-ycf1_p5	AAAAAGAAATCCTTCCCTTTTT	TTGAACCTGTCCGTTTGTCT	27.3	45.0	52.6	57.3	845-859 bp	1.0	43.7