Hotspot

rpl32-trnL-CUA

SSC · 114002-114787 · 786 bp

Back to Boechera

Pi 0.0188

MarkerSeek score 71.70

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 786
variable_sites: 34
pi: 0.0188
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	846-927 bp	1.0	71.8
rpl32-trnL-CUA_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	839-920 bp	1.0	71.4
rpl32-trnL-CUA_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	845-926 bp	1.0	71.3
rpl32-trnL-CUA_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	844-925 bp	1.0	71.3
rpl32-trnL-CUA_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	840-921 bp	1.0	70.7