Hotspot

psaJ-rpl33

LSC · 67110-67532 · 423 bp

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Pi 0.0214

MarkerSeek score 52.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 18
pi: 0.0214
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1103-1115 bp	1.0	82.4
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.1	58.1	1115-1127 bp	1.0	82.3
psaJ-rpl33_p3	TACCTCGTTACTGCCCAGAA	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1155-1167 bp	0.666667	68.4
psaJ-rpl33_p4	TACCTCGTTACTGCCCAGAA	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.1	58.1	1167-1179 bp	0.666667	68.4
psaJ-rpl33_p5	TACCTCGTTACTGCCCAGAA	TTCCAATCGACTAGGGGTGT	50.0	50.0	58.1	58.1	1168-1180 bp	0.666667	68.4