Hotspot

rbcL-accD

LSC · 56447-57126 · 680 bp

Back to Boechera

Pi 0.0163
MarkerSeek score 50.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
680
variable_sites
20
pi
0.0163
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AATGAGGGACGTGATCTTGC ATTCCGAATGTCCTTGGAGC 50.0 50.0 58.0 58.0 1034-1053 bp 1.0 82.0
rbcL-accD_p2 TGCAATGCACGCTGTTATTG ATTCCGAATGTCCTTGGAGC 45.0 50.0 58.0 58.0 1443-1462 bp 1.0 81.5
rbcL-accD_p3 TTGAATGCTACTGCGGGTAC ATTCCGAATGTCCTTGGAGC 50.0 50.0 58.0 58.0 1610-1629 bp 1.0 81.3
rbcL-accD_p4 CTTAACAGGGGGATTCACCG ATTCCGAATGTCCTTGGAGC 55.0 50.0 58.0 58.0 1521-1540 bp 1.0 81.2
rbcL-accD_p5 TCATGGTATGCACTTCCGTG ATTCCGAATGTCCTTGGAGC 50.0 50.0 58.0 58.0 1414-1429 bp 0.833333 74.5