Hotspot

trnT-ACA-trnL-UUA

LSC · 46290-47053 · 764 bp

Back to Boechera

Pi 0.014

MarkerSeek score 68.40

Primer available yes

Label trnT-ACA-trnL-UUA

Feature Metadata

label_name: trnT-ACA-trnL-UUA
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 24
pi: 0.014
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACA-trnL-UUA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	802-843 bp	1.0	82.7
trnT-ACA-trnL-UUA_p2	ACAATATGGAAAAGCCGGCT	CCGTAGCGTCTACCAATTCC	45.0	55.0	57.9	58.2	857-898 bp	1.0	82.3
trnT-ACA-trnL-UUA_p3	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	853-894 bp	1.0	82.1
trnT-ACA-trnL-UUA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	805-846 bp	1.0	82.0
trnT-ACA-trnL-UUA_p5	ACAATATGGAAAAGCCGGCT	AGTCCGTAGCGTCTACCAAT	45.0	50.0	57.9	58.2	860-901 bp	1.0	81.6