Hotspot

psbZ-trnG-GGC

LSC · 35999-36475 · 477 bp

Back to Boechera

Pi 0.00914
MarkerSeek score 64.40
Primer available yes
Label psbZ-trnG-GGC

Feature Metadata

label_name
psbZ-trnG-GGC
feature_type
igs
region
LSC
length_bp
477
variable_sites
9
pi
0.00914
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GGC_p1 TCCTGATGGTTGGTCGAGTA CGGATAGCGGGAATCGAAC 50.0 57.9 57.8 58.2 642-652 bp 1.0 71.0
psbZ-trnG-GGC_p2 TCCTGATGGTTGGTCGAGTA GATAGCGGGAATCGAACCC 50.0 57.9 57.8 57.8 640-650 bp 1.0 70.9
psbZ-trnG-GGC_p3 TCCTGATGGTTGGTCGAGTA GGATAGCGGGAATCGAACC 50.0 57.9 57.8 57.8 641-651 bp 1.0 70.9
psbZ-trnG-GGC_p4 CCGTTGTATTTGCGTCTCCT CGGATAGCGGGAATCGAAC 50.0 57.9 58.3 58.2 658-668 bp 1.0 70.6
psbZ-trnG-GGC_p5 CCGTTGTATTTGCGTCTCCT GATAGCGGGAATCGAACCC 50.0 57.9 58.3 57.8 656-666 bp 1.0 70.4