Hotspot

psbM-trnD-GAC

LSC · 28874-29889 · 1016 bp

Back to Boechera

Pi 0.0118

MarkerSeek score 59.20

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 25
pi: 0.0118
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	AGGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1125-1134 bp	1.0	70.4
psbM-trnD-GAC_p2	ACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.9	58.5	1121-1130 bp	1.0	69.7
psbM-trnD-GAC_p3	GGAACGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.3	58.5	1124-1133 bp	1.0	68.2
psbM-trnD-GAC_p4	AACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.6	58.5	1122-1131 bp	1.0	67.5
psbM-trnD-GAC_p5	GGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.5	58.5	1124-1133 bp	1.0	67.0