Hotspot

rpoB-trnC-UGC

LSC · 26324-27423 · 1100 bp

Back to Boechera

Pi 0.0105

MarkerSeek score 55.30

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1100
variable_sites: 25
pi: 0.0105
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1188-1189 bp	1.0	80.5
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1170-1171 bp	1.0	78.4
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	AAAGTCTGTTGTTGAGGCGG	55.0	50.0	57.9	58.7	1211-1212 bp	1.0	75.6
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1189-1190 bp	1.0	74.5
rpoB-trnC-UGC_p5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	55.0	52.6	57.9	58.0	1210-1211 bp	1.0	73.1