Hotspot

atpH-atpI

LSC · 13500-13997 · 498 bp

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Pi 0.0102

MarkerSeek score 61.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 11
pi: 0.0102
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	602-609 bp	1.0	81.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	660-667 bp	1.0	81.1
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	642-649 bp	1.0	81.0
atpH-atpI_p4	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	664-671 bp	1.0	80.4
atpH-atpI_p5	AACCGAAGCAGCAGAAATCA	ACCTGTCATGTTCCTGGGAT	45.0	50.0	58.1	58.3	626-633 bp	1.0	78.8