Hotspot

trnR-AGA-atpA

LSC · 9567-9847 · 281 bp

Back to Boechera

Pi 0.0135

MarkerSeek score 58.90

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 9
pi: 0.0135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	GCGAAAAGCGTCCATTGTC	TCATAGCCTCGACCAAGACA	52.6	50.0	58.0	58.2	461-470 bp	1.0	72.3
trnR-AGA-atpA_p2	GCGAAAAGCGTCCATTGTC	CGACCAAGACATTAACCGCT	52.6	50.0	58.0	58.3	452-461 bp	1.0	71.3
trnR-AGA-atpA_p3	GCGAAAAGCGTCCATTGTC	TCGACCAAGACATTAACCGC	52.6	50.0	58.0	58.3	453-462 bp	1.0	71.3
trnR-AGA-atpA_p4	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	52.6	45.0	58.0	57.7	403-412 bp	1.0	71.2
trnR-AGA-atpA_p5	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	52.6	45.0	58.0	57.7	404-413 bp	1.0	71.1