Hotspot

trnH-CAC-psbA

LSC · 76-379 · 304 bp

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Pi 0.03

MarkerSeek score 65.30

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 304
variable_sites: 21
pi: 0.03
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	366-391 bp	1.0	87.1
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	369-394 bp	1.0	87.0
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	783-808 bp	1.0	82.7
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	CTGCGACTGCTGTTTTCTTG	50.0	50.0	57.9	58.0	926-951 bp	1.0	82.3
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CTGCGACTGCTGTTTTCTTG	50.0	50.0	58.2	58.0	927-952 bp	1.0	82.1