Hotspot

rbcL

LSC · 58891-60318 · 1428 bp

Back to Bobgunnia

Pi 0.0021

MarkerSeek score 60.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 3
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGACCTTGTTGTTGCGAGAA	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.7	58.4	1526 bp	1.0	74.5
rbcL_p2	AGACCTTGTTGTTGCGAGAA	TTGGGCCGAGTTTAATTGCA	45.0	45.0	57.7	58.4	1527 bp	1.0	74.5
rbcL_p3	AGTAGACCTTGTTGTTGCGA	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.1	58.4	1529 bp	1.0	69.9
rbcL_p4	AGTAGACCTTGTTGTTGCGA	TTGGGCCGAGTTTAATTGCA	45.0	45.0	57.1	58.4	1530 bp	1.0	69.9
rbcL_p5	AGACCTTGTTGTTGCGAGAA	GGGCCGAGTTTAATTGCAAT	45.0	45.0	57.7	57.0	1525 bp	1.0	69.9