Hotspot

ndhG-ndhI

SSC · 124404-124951 · 548 bp

Back to Bobgunnia

Pi 0.00377
MarkerSeek score 52.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
548
variable_sites
2
pi
0.00377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAATCATGTAGCGGTTCGGG TGCTTTGGGACGTTTACCAA 50.0 45.0 58.3 57.9 659-665 bp 1.0 79.3
ndhG-ndhI_p2 AAATCATGTAGCGGTTCGGG TTGCTTTGGGACGTTTACCA 50.0 45.0 58.3 57.9 660-666 bp 1.0 79.3
ndhG-ndhI_p3 AAATCATGTAGCGGTTCGGG TGGGACGTTTACCAATGTCA 50.0 45.0 58.3 57.1 654-660 bp 1.0 72.3
ndhG-ndhI_p4 GAAAATCATGTAGCGGTTCGG TGCTTTGGGACGTTTACCAA 47.6 45.0 57.7 57.9 661-667 bp 1.0 71.4
ndhG-ndhI_p5 GAAAATCATGTAGCGGTTCGG TTGCTTTGGGACGTTTACCA 47.6 45.0 57.7 57.9 662-668 bp 1.0 71.4