Hotspot

rpl32-trnL-UAG

SSC · 118903-119714 · 812 bp

Back to Bobgunnia

Pi 0.00127

MarkerSeek score 62.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 812
variable_sites: 1
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCGCAATCTCTTTCTACGG	GATAAGATGCCATGCCGCTA	50.0	50.0	58.1	57.9	946-971 bp	1.0	81.0
rpl32-trnL-UAG_p2	AGCGCAATCTCTTTCTACGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	893-918 bp	1.0	80.9
rpl32-trnL-UAG_p3	AGCGCAATCTCTTTCTACGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.1	57.8	886-911 bp	1.0	80.4
rpl32-trnL-UAG_p4	AGCGCAATCTCTTTCTACGG	ATAAGATGCCATGCCGCTAC	50.0	50.0	58.1	58.2	945-970 bp	1.0	80.3
rpl32-trnL-UAG_p5	AGCGCAATCTCTTTCTACGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.1	57.8	891-916 bp	1.0	80.3