Hotspot

ndhF-rpl32

SSC · 117740-118734 · 995 bp

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Pi 0.00202
MarkerSeek score 60.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
995
variable_sites
2
pi
0.00202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCGTGGGAAAAAGTA AAAGCTTTCAATGTTGCCCA 45.0 40.0 57.9 56.7 1185-1189 bp 1.0 69.6
ndhF-rpl32_p2 TGTTGCCGTGGGAAAAAGTA AAGCTTTCAATGTTGCCCAA 45.0 40.0 57.9 56.7 1184-1188 bp 1.0 69.6
ndhF-rpl32_p3 AAAGTATAAGTCCCGCCCCT AAAGCTTTCAATGTTGCCCA 50.0 40.0 58.1 56.7 1171-1175 bp 1.0 69.1
ndhF-rpl32_p4 AAAGTATAAGTCCCGCCCCT AAGCTTTCAATGTTGCCCAA 50.0 40.0 58.1 56.7 1170-1174 bp 1.0 69.1
ndhF-rpl32_p5 TGTTGCCGTGGGAAAAAGTA TGTTGCCCAATATCCCTTCT 45.0 45.0 57.9 56.5 1174-1178 bp 1.0 67.8