Hotspot

ycf1-ndhF

SSC · 115198-115492 · 295 bp

Back to Bobgunnia

Pi 0.057

MarkerSeek score 69.20

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 295
variable_sites: 13
pi: 0.057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCTCAAGGGACCCAAAAGAA	CGTTGGGGCCATAGGAATTT	50.0	50.0	58.0	58.2	1060-1120 bp	1.0	83.2
ycf1-ndhF_p2	TTCTCCATGGGGCCTTCTTA	CGTTGGGGCCATAGGAATTT	50.0	50.0	58.0	58.2	1096-1156 bp	1.0	83.0
ycf1-ndhF_p3	CTGACCACATTCTCCATGGG	CGTTGGGGCCATAGGAATTT	55.0	50.0	58.0	58.2	1105-1165 bp	1.0	82.8
ycf1-ndhF_p4	TCTTCCTTCTCCGAGCTCAA	CGTTGGGGCCATAGGAATTT	50.0	50.0	58.1	58.2	1074-1134 bp	1.0	82.6
ycf1-ndhF_p5	AACCCACGACCAATATGAGC	CGTTGGGGCCATAGGAATTT	50.0	50.0	58.0	58.2	1516-1576 bp	1.0	82.1