Hotspot

rps3-rps19

LSC · 89035-89630 · 596 bp

Back to Bobgunnia

Pi 0.00177

MarkerSeek score 64.50

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 1
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	TAAACTTCCGAGGACATGCG	50.0	50.0	58.4	58.0	650-680 bp	1.0	80.4
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	50.0	45.0	58.4	57.9	644-674 bp	1.0	79.6
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	50.0	45.0	58.4	58.1	645-675 bp	1.0	79.6
rps3-rps19_p4	AGTCTGAAACCAAGCGGATT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	648-678 bp	1.0	79.2
rps3-rps19_p5	AAGTCTGAAACCAAGCGGAT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	649-679 bp	1.0	79.1