Hotspot

rps8-rpl14

LSC · 85560-86128 · 569 bp

Back to Bobgunnia

Pi 0.00473

MarkerSeek score 59.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 2
pi: 0.00473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGTCAGCGTTTCGTATCGAG	GAACTCGAATTTTTGGCGCA	50.0	45.0	58.1	58.0	561-707 bp	1.0	83.0
rps8-rpl14_p2	TGTCAGCGTTTCGTATCGAG	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.1	57.9	502-648 bp	1.0	82.5
rps8-rpl14_p3	TTATGTCAGCGATGGTGTCC	GAACTCGAATTTTTGGCGCA	50.0	45.0	57.7	58.0	537-683 bp	1.0	81.4
rps8-rpl14_p4	GTCAGCGATGGTGTCCTTAC	GAACTCGAATTTTTGGCGCA	55.0	45.0	58.4	58.0	533-679 bp	1.0	81.0
rps8-rpl14_p5	TTATGTCAGCGATGGTGTCC	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.7	57.9	478-624 bp	1.0	80.9