Hotspot

rbcL-accD

LSC · 60319-61722 · 1404 bp

Back to Bobgunnia

Pi 0.00297
MarkerSeek score 64.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1404
variable_sites
4
pi
0.00297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTCCTGAATTAGCTGCTGC GGTCTTCGCTTACACTGGTA 50.0 50.0 58.0 57.0 1558-1597 bp 1.0 74.6
rbcL-accD_p2 GAGGCTAGCAAATGGAGTCC GGTCTTCGCTTACACTGGTA 55.0 50.0 58.0 57.0 1573-1612 bp 1.0 74.5
rbcL-accD_p3 AGTCCTGAATTAGCTGCTGC GGGTCTTCGCTTACACTGG 50.0 57.9 58.0 57.9 1559-1598 bp 1.0 73.6
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC GGGTCTTCGCTTACACTGG 55.0 57.9 58.0 57.9 1574-1613 bp 1.0 73.6
rbcL-accD_p5 AGTCCTGAATTAGCTGCTGC CGGGTCTTCGCTTACACTG 50.0 57.9 58.0 58.3 1560-1599 bp 1.0 72.7