Hotspot

ndhC-trnV-UAC

LSC · 54370-55103 · 734 bp

Back to Bobgunnia

Pi 0.00411

MarkerSeek score 61.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 3
pi: 0.00411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	877-887 bp	1.0	76.3
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	876-886 bp	1.0	73.6
ndhC-trnV-UAC_p3	CTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	884-894 bp	1.0	73.5
ndhC-trnV-UAC_p4	TCTCTGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	885-895 bp	1.0	73.3
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	865-875 bp	1.0	72.4