Hotspot

atpF-atpH

LSC · 14492-15174 · 683 bp

Back to Bobgunnia

Pi 0.00587

MarkerSeek score 62.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 4
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAATCCGAAACTCCCGGC	TGGGCTGGTTGTAGCATTAG	50.0	50.0	58.3	57.6	800-807 bp	1.0	75.2
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	AGGCAGAGGGAAAAATACGG	50.0	50.0	58.3	57.6	867-874 bp	1.0	75.1
atpF-atpH_p3	CCAGTAACCCAAGCAAAGGA	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.7	57.6	774-781 bp	1.0	75.0
atpF-atpH_p4	CCAGTAACCCAAGCAAAGGA	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.7	57.6	841-848 bp	1.0	74.9
atpF-atpH_p5	TTAAATCCGAAACTCCCGGC	GCAGAGGGAAAAATACGGGG	50.0	55.0	58.3	58.6	865-872 bp	1.0	73.6