Hotspot

trnS-GGA-trnG-GCC

LSC · 8848-9894 · 1047 bp

Back to Bobgunnia

Pi 0.00194

MarkerSeek score 61.30

Primer available yes

Label trnS-GGA-trnG-GCC

Feature Metadata

label_name: trnS-GGA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1047
variable_sites: 2
pi: 0.00194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1144-1159 bp	1.0	75.5
trnS-GGA-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1143-1158 bp	1.0	75.5
trnS-GGA-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.2	57.3	1197-1212 bp	1.0	74.3
trnS-GGA-trnG-GCC_p4	AGCAATCCGACGCTTTAGTC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.4	57.3	1146-1161 bp	1.0	72.7
trnS-GGA-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	GAGTCCATAGAACGAATCGCA	50.0	47.6	58.0	57.9	1112-1127 bp	1.0	72.6