Hotspot

trnK-UUU-rps16

LSC · 4496-5270 · 775 bp

Back to Bobgunnia

Pi 0.00876

MarkerSeek score 66.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 6
pi: 0.00876
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	TATTTCCTCGAACAAGGCGC	50.0	50.0	57.9	58.6	910-999 bp	1.0	78.9
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	TATTTCCTCGAACAAGGCGC	50.0	50.0	57.9	58.6	909-998 bp	1.0	78.9
trnK-UUU-rps16_p3	CAGTCGCGGTCTTACAAACT	TATTTCCTCGAACAAGGCGC	50.0	50.0	58.2	58.6	911-1000 bp	1.0	77.7
trnK-UUU-rps16_p4	TCAGTCGCGGTCTTACAAAC	TATTTCCTCGAACAAGGCGC	50.0	50.0	58.2	58.6	912-1001 bp	1.0	77.6
trnK-UUU-rps16_p5	GATCAGTCGCGGTCTTACAA	TATTTCCTCGAACAAGGCGC	50.0	50.0	57.7	58.6	914-1003 bp	1.0	77.2