Hotspot

ycf1

IRb · 113097-113603 · 507 bp

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Pi 0
MarkerSeek score 56.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
507
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GAGCTACTGAGGAACAACGG GTTGACTCCACCGGAAAACT 55.0 50.0 58.0 58.0 1528 bp 1.0 79.7
ycf1_p2 GAGCTACTGAGGAACAACGG TCCACTCTTTATGGGTCCGT 55.0 50.0 58.0 58.1 1410 bp 1.0 79.6
ycf1_p3 TGTAATGGCTCCGTTCCATG GTTGACTCCACCGGAAAACT 50.0 50.0 58.0 58.0 1420 bp 1.0 79.4
ycf1_p4 GCCAGGTATTGACGGTGATT GTTGACTCCACCGGAAAACT 50.0 50.0 58.0 58.0 1749 bp 1.0 79.4
ycf1_p5 TGTAATGGCTCCGTTCCATG TCCACTCTTTATGGGTCCGT 50.0 50.0 58.0 58.1 1302 bp 1.0 79.3