Hotspot

rps16

LSC · 4609-5915 · 1307 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1307
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACCACCCTAATCCACTTTTGA TGATTCCGTACGGATTCAAA 42.9 40.0 56.8 54.8 1370 bp 1.0 40.0
rps16_p2 ACCACCCTAATCCACTTTTGA TTGAATTGATTCCGTACGGA 42.9 40.0 56.8 54.8 1376 bp 1.0 40.0
rps16_p3 ACCACCCTAATCCACTTTTGA TGAATTGATTCCGTACGGATTCA 42.9 39.1 56.8 58.2 1375 bp 1.0 40.0
rps16_p4 ACCACCCTAATCCACTTTTGA CCGTACGGATTCAAAATTTGAGG 42.9 43.5 56.8 58.3 1365 bp 1.0 40.0
rps16_p5 ACCACCCTAATCCACTTTTGA TGATTCCGTACGGATTCAAAA 42.9 38.1 56.8 55.6 1370 bp 1.0 40.0