Hotspot

matK

LSC · 1831-3345 · 1515 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1515
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACACAGTTTCATACCCAATCA AGGCTTCTGTCTCTAGCTCA 38.1 50.0 55.1 57.5 1585 bp 1.0 44.7
matK_p2 ACACAGTTTCATACCCAATCATG AGGCTTCTGTCTCTAGCTCA 39.1 50.0 57.1 57.5 1585 bp 1.0 44.6
matK_p3 CACAGTTTCATACCCAATCATGA AGGCTTCTGTCTCTAGCTCA 39.1 50.0 56.8 57.5 1584 bp 1.0 42.6
matK_p4 GCATGAGCATTTCAGTAGAA AGGCTTCTGTCTCTAGCTCA 40.0 50.0 53.7 57.5 1668 bp 1.0 41.4
matK_p5 ACACAGTTTCATACCCAATCAT AGGCTTCTGTCTCTAGCTCA 36.4 50.0 55.7 57.5 1585 bp 1.0 41.3