Hotspot

rps16-trnQ-UUG

LSC · 5916-6733 · 818 bp

Back to Blyxa

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
818
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TGGTCCTGTTATTCGGAGGT 45.0 50.0 58.1 58.1 906 bp 1.0 78.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 891 bp 1.0 78.3
rps16-trnQ-UUG_p3 GTGATGTCCTTCAAGTCGCA TGGTCCTGTTATTCGGAGGT 50.0 50.0 58.2 58.1 918 bp 1.0 77.9
rps16-trnQ-UUG_p4 TGATGTCCTTCAAGTCGCAC TGGTCCTGTTATTCGGAGGT 50.0 50.0 58.2 58.1 917 bp 1.0 77.9
rps16-trnQ-UUG_p5 TGATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 902 bp 1.0 77.8