Hotspot

matK-trnK-UUU

LSC · 3346-4076 · 731 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
731
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 GGAGGTGTTGTTGCCGATAT TCTGGGTTGCTAACTCAACG 50.0 50.0 58.0 57.8 826 bp 1.0 78.4
matK-trnK-UUU_p2 GGAGGTGTTGTTGCCGATAT AACGGTAGAGTACTCGGCTT 50.0 50.0 58.0 58.2 810 bp 1.0 78.3
matK-trnK-UUU_p3 GGAGGTGTTGTTGCCGATAT ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.0 58.2 809 bp 1.0 78.3
matK-trnK-UUU_p4 GGAGGTGTTGTTGCCGATAT GGGTTGCTAACTCAACGGTA 50.0 50.0 58.0 57.5 823 bp 1.0 76.0
matK-trnK-UUU_p5 TATAGGAGGTGTTGTTGCCG TCTGGGTTGCTAACTCAACG 50.0 50.0 57.3 57.8 830 bp 1.0 73.2