Hotspot

rps16

LSC · 5199-6332 · 1134 bp

Back to Blumea

Pi 0.00938
MarkerSeek score 55.60
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1134
variable_sites
27
pi
0.00938
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TCCTACCCTTAGTTTTTCTTTCCT ACCGACTGAACCAATGACTA 37.5 45.0 57.2 56.2 1220-1240 bp 0.875 39.4
rps16_p2 TCCTACCCTTAGTTTTTCTTTCCT TACCGACTGAACCAATGACT 37.5 45.0 57.2 56.2 1221-1241 bp 0.875 39.4
rps16_p3 TCCTACCCTTAGTTTTTCTTTCC ACCGACTGAACCAATGACTA 39.1 45.0 55.8 56.2 1220-1240 bp 0.875 39.4
rps16_p4 TCCTACCCTTAGTTTTTCTTTCC TACCGACTGAACCAATGACT 39.1 45.0 55.8 56.2 1221-1241 bp 0.875 39.4
rps16_p5 TCCTACCCTTAGTTTTTCTTTCCTT ACCGACTGAACCAATGACTA 36.0 45.0 57.8 56.2 1220-1240 bp 0.875 39.4