Hotspot

ndhF

SSC · 107869-110097 · 2229 bp

Pi 0.00975

MarkerSeek score 45.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 54
pi: 0.00975
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	AGACCGAGGACCTTAGTGAA	55.0	50.0	57.9	57.4	2903-2952 bp	1.0	77.7
ndhF_p2	TGTTTCGATGCAACAGCAAG	AGACCGAGGACCTTAGTGAA	45.0	50.0	57.6	57.4	2677-2726 bp	1.0	75.9
ndhF_p3	CTAGCACTGGGTAGACCTCA	CCGAGGACCTTAGTGAATCC	55.0	55.0	57.9	57.1	2900-2949 bp	1.0	75.4
ndhF_p4	TCTAGCACTGGGTAGACCTC	AGACCGAGGACCTTAGTGAA	55.0	50.0	57.6	57.4	2904-2953 bp	1.0	75.4
ndhF_p5	AACTGTCCTAGCTCTACCGT	AGACCGAGGACCTTAGTGAA	50.0	50.0	57.5	57.4	2877-2926 bp	1.0	74.8