Hotspot

ndhD

SSC · 113591-115093 · 1503 bp

Pi 0.00725

MarkerSeek score 46.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 26
pi: 0.00725
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGTCTCAACTGGCGAGAAC	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	1942-1949 bp	1.0	81.4
ndhD_p2	CTTGTCTCAACTGGCGAGAA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.8	58.0	1943-1950 bp	1.0	81.2
ndhD_p3	TCAGGAGCAGTATGGGCTAA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.8	58.0	2252-2259 bp	1.0	80.8
ndhD_p4	TTCAGGAGCAGTATGGGCTA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.8	58.0	2253-2260 bp	1.0	80.8
ndhD_p5	TTGTCTCAACTGGCGAGAAC	AAAACTCGGGGAACTCATCG	50.0	50.0	58.1	57.9	2170-2177 bp	1.0	80.6