Hotspot

rps15-ycf1

SSC · 121394-121793 · 400 bp

Back to Blumea

Pi 0.0209
MarkerSeek score 60.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
400
variable_sites
19
pi
0.0209
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CCGCAGACCTCTCTGAGATA AATTATGGAACCTGCCCGTC 55.0 50.0 58.0 57.9 1073-1097 bp 1.0 83.9
rps15-ycf1_p2 CCGCAGACCTCTCTGAGATA TATGGAACCTGCCCGTCTAT 55.0 50.0 58.0 57.9 1070-1094 bp 1.0 83.6
rps15-ycf1_p3 AACACCATGCATTGATGGGT AATTATGGAACCTGCCCGTC 45.0 50.0 58.1 57.9 1350-1374 bp 1.0 83.3
rps15-ycf1_p4 AACACCATGCATTGATGGGT TATGGAACCTGCCCGTCTAT 45.0 50.0 58.1 57.9 1347-1371 bp 1.0 82.9
rps15-ycf1_p5 AATAAGCCAGCAACCGTTGA AATTATGGAACCTGCCCGTC 45.0 50.0 58.1 57.9 1112-1134 bp 0.875 77.6