Hotspot

ndhG-ndhI

SSC · 116744-117096 · 353 bp

Back to Blumea

Pi 0.023
MarkerSeek score 58.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
353
variable_sites
20
pi
0.023
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TACTACTCCCAGACCCCCTA TCAAATTGCTTTGGGTCGGT 55.0 45.0 57.7 58.3 503-522 bp 1.0 82.2
ndhG-ndhI_p2 ATACTACTCCCAGACCCCCT TCAAATTGCTTTGGGTCGGT 55.0 45.0 58.4 58.3 504-523 bp 1.0 81.8
ndhG-ndhI_p3 AATACTACTCCCAGACCCCC TCAAATTGCTTTGGGTCGGT 55.0 45.0 57.5 58.3 505-524 bp 1.0 80.8
ndhG-ndhI_p4 TACTACTCCCAGACCCCCTA CAAATTGCTTTGGGTCGGTT 55.0 45.0 57.7 57.5 502-521 bp 1.0 80.5
ndhG-ndhI_p5 ATACTACTCCCAGACCCCCT CAAATTGCTTTGGGTCGGTT 55.0 45.0 58.4 57.5 503-522 bp 1.0 80.0