Hotspot

rpl32-trnL-UAG

SSC · 111318-112176 · 859 bp

Back to Blumea

Pi 0.0188
MarkerSeek score 56.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
859
variable_sites
40
pi
0.0188
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 974-991 bp 1.0 81.7
rpl32-trnL-UAG_p2 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 975-992 bp 1.0 81.7
rpl32-trnL-UAG_p3 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 991-1008 bp 0.625 65.7
rpl32-trnL-UAG_p4 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 992-1009 bp 0.625 65.7
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TGATCCACAAAGAAGAGCCG 50.0 50.0 57.6 57.9 1612-1629 bp 0.625 64.0