Hotspot

ndhF-rpl32

SSC · 110098-111152 · 1055 bp

Back to Blumea

Pi 0.0192
MarkerSeek score 56.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1055
variable_sites
50
pi
0.0192
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.0 55.3 1193-1214 bp 1.0 44.8
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1191-1212 bp 1.0 44.8
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1192-1213 bp 1.0 44.8
ndhF-rpl32_p4 CTTTTTGTTGCCGTCGGAAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1199-1220 bp 1.0 44.8
ndhF-rpl32_p5 TTTGTTGCCGTCGGAAAAAG TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1196-1217 bp 1.0 44.8