Hotspot

ycf2-trnL-CAA

IRb · 91934-92348 · 415 bp

Back to Blumea

Pi 0.0203
MarkerSeek score 59.80
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
415
variable_sites
17
pi
0.0203
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 GCTTTTCCCGGATGAAATGC AAATGGTAGACACGCGAGAC 50.0 50.0 58.1 58.0 569 bp 1.0 81.7
ycf2-trnL-CAA_p2 GCTTTTCCCGGATGAAATGC TGAAATGGTAGACACGCGAG 50.0 50.0 58.1 58.0 571 bp 1.0 81.7
ycf2-trnL-CAA_p3 GCTTTTCCCGGATGAAATGC GCCTTGGTGGTGAAATGGTA 50.0 50.0 58.1 58.1 581 bp 1.0 81.1
ycf2-trnL-CAA_p4 ATGGCTTTTCCCGGATGAAA AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 572 bp 1.0 80.5
ycf2-trnL-CAA_p5 TGGCTTTTCCCGGATGAAAT AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 571 bp 1.0 80.5