Hotspot

accD-psaI

LSC · 57585-58303 · 719 bp

Back to Blumea

Pi 0.0143
MarkerSeek score 56.80
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
719
variable_sites
30
pi
0.0143
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CACACTTTCTTTCCTTTGAATCA GGGACCAAAACAGAGGGAAA 34.8 50.0 55.6 57.6 775-833 bp 1.0 45.3
accD-psaI_p2 CACACTTTCTTTCCTTTGAATCA AGGCCTACTAAAGGGACCAA 34.8 50.0 55.6 57.6 787-845 bp 1.0 45.2
accD-psaI_p3 CACACTTTCTTTCCTTTGAATCA TTGCGATTGCCGGAAATACT 34.8 45.0 55.6 58.3 807-865 bp 1.0 45.1
accD-psaI_p4 CACACTTTCTTTCCTTTGAATCA AAAGAAGCCATTGCGATTGC 34.8 45.0 55.6 58.0 817-875 bp 1.0 45.0
accD-psaI_p5 CACACTTTCTTTCCTTTGAATCAA AAAGAAGCCATTGCGATTGC 33.3 45.0 56.3 58.0 817-875 bp 1.0 45.0