Hotspot

ndhC-trnV-UAC

LSC · 50019-50356 · 338 bp

Back to Blumea

Pi 0.0345
MarkerSeek score 52.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
338
variable_sites
26
pi
0.0345
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.75
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCGTCGCCTATTGGTTCTAT ATGAAAGAGCCCAATGCACA 50.0 45.0 57.8 58.1 510-622 bp 1.0 86.6
ndhC-trnV-UAC_p2 GCGTCGCCTATTGGTTCTAT GTCCAGTTAGGTGCCAAACA 50.0 50.0 57.8 58.0 864-976 bp 1.0 85.8
ndhC-trnV-UAC_p3 GCGTCGCCTATTGGTTCTAT GTGTCCAGTTAGGTGCCAAA 50.0 50.0 57.8 58.0 866-978 bp 1.0 85.8
ndhC-trnV-UAC_p4 GCGTCGCCTATTGGTTCTAT TGTCCAGTTAGGTGCCAAAC 50.0 50.0 57.8 58.0 865-977 bp 1.0 85.8
ndhC-trnV-UAC_p5 ACATCGAAACTCATTGCCCA GTCCAGTTAGGTGCCAAACA 45.0 50.0 57.8 58.0 966-1078 bp 1.0 85.1