Hotspot

trnE-UUC-rpoB

LSC · 12145-13037 · 893 bp

Back to Blumea

Pi 0.015
MarkerSeek score 61.00
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
893
variable_sites
33
pi
0.015
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGACTTGAACCCTCGTTTT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.0 56.8 1035-1081 bp 1.0 65.6
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 994-1040 bp 1.0 65.1
trnE-UUC-rpoB_p3 CCCTCGTTTTCTCCGTGAAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1025-1071 bp 1.0 64.9
trnE-UUC-rpoB_p4 TTGAACCCTCGTTTTCTCCG AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1030-1076 bp 1.0 64.8
trnE-UUC-rpoB_p5 GGGACTTGAACCCTCGTTTT TCAAACCCTGATCAATGAACCT 50.0 40.9 58.0 57.6 1025-1071 bp 1.0 64.3